Vieira A. auteur aut IRD Talhinhas P. auteur aut IRD Loureiro A. auteur aut IRD Thurich J. auteur aut IRD Duplessis S. auteur aut IRD Fernandez Diana auteur aut IRD Do Ceu Silva M. auteur aut IRD Paulo O. S. auteur aut IRD Azinheira H. G. auteur aut IRD text journalArticle eng text/pdf born digital access Coffee Leaf Rust, caused by Hemileia vastatrix, is the most important disease of Arabica coffee (Coffea arabica), which prompts studies aimed at understanding the genetic basis of this pathogen as well as its complex interaction with the host. In this work, 11 genes, putatively involved in signalling, establishment and maintenance of biotrophy (transport and metabolism), were characterised, and their expression profiles during host infection were assessed by RT-qPCR in three compatible coffee-rust interactions comprising two different rust races. The profiles of two chitin deacetylases (CD) and a heterotrimeric G-protein alpha subunit transcripts suggest that these enzymes are involved in host-pathogen recognition and establishment of biotrophy at early stages of infection, and the late expression of the CD1 gene was also recorded. Different expression profiles were observed for a MAP kinase gene between the two rust races, suggesting that this gene may be involved in the differentiation of infection structures in a race-specific pattern. Two amino acid transporters, an invertase, a hexose transporter and a mannitol dehydrogenase presented expression profiles similar to those reported in other rust fungi, indicating a fairly conserved genetic programme related to host infection in rust fungi. The strong upregulation of a Uromyces fabae rust transferred protein 1 orthologous gene was observed in H. vastatrix in planta structures, suggesting that this gene may also play a role during the establishment and the maintenance of biotrophy in coffee leaves. Overall, our results provide valuable insights to the current understanding of the biotrophic interaction between H. vastatrix - C. arabica at the molecular level and will contribute to a reasoned and sustainable use of resistant genotypes. specialized Coffee leaf rust Reverse transcription quantitative PCR Transcript profiling Pucciniales 076 133 1 261-277 2012 0929-1873 https://www.documentation.ird.fr/hor/fdi:010055815 10.1007/s10658-011-9864-5 0929-1873 [F B010055815] https://www.documentation.ird.fr/hor/fdi:010055815 https://www.documentation.ird.fr/intranet/publi/2012/05/010055815.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-06-01 2017-08-23 fdi:010055815 fre