@article{fdi:010055815,
  title = {{E}xpression profiling of genes involved in the biotrophic colonisation of {C}offea arabica leaves by {H}emileia vastatrix},
  author = {{V}ieira, {A}. and {T}alhinhas, {P}. and {L}oureiro, {A}. and {T}hurich, {J}. and {D}uplessis, {S}. and {F}ernandez, {D}iana and {D}o {C}eu {S}ilva, {M}. and {P}aulo, {O}. {S}. and {A}zinheira, {H}. {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}offee {L}eaf {R}ust, caused by {H}emileia vastatrix, is the most important disease of {A}rabica coffee ({C}offea arabica), which prompts studies aimed at understanding the genetic basis of this pathogen as well as its complex interaction with the host. {I}n this work, 11 genes, putatively involved in signalling, establishment and maintenance of biotrophy (transport and metabolism), were characterised, and their expression profiles during host infection were assessed by {RT}-q{PCR} in three compatible coffee-rust interactions comprising two different rust races. {T}he profiles of two chitin deacetylases ({CD}) and a heterotrimeric {G}-protein alpha subunit transcripts suggest that these enzymes are involved in host-pathogen recognition and establishment of biotrophy at early stages of infection, and the late expression of the {CD}1 gene was also recorded. {D}ifferent expression profiles were observed for a {MAP} kinase gene between the two rust races, suggesting that this gene may be involved in the differentiation of infection structures in a race-specific pattern. {T}wo amino acid transporters, an invertase, a hexose transporter and a mannitol dehydrogenase presented expression profiles similar to those reported in other rust fungi, indicating a fairly conserved genetic programme related to host infection in rust fungi. {T}he strong upregulation of a {U}romyces fabae rust transferred protein 1 orthologous gene was observed in {H}. vastatrix in planta structures, suggesting that this gene may also play a role during the establishment and the maintenance of biotrophy in coffee leaves. {O}verall, our results provide valuable insights to the current understanding of the biotrophic interaction between {H}. vastatrix - {C}. arabica at the molecular level and will contribute to a reasoned and sustainable use of resistant genotypes.},
  keywords = {{C}offee leaf rust ; {R}everse transcription quantitative {PCR} ; {T}ranscript ; profiling ; {P}ucciniales},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {P}lant {P}athology},
  volume = {133},
  numero = {1},
  pages = {261--277},
  ISSN = {0929-1873},
  year = {2012},
  DOI = {10.1007/s10658-011-9864-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055815},
}