@article{fdi:010055769, title = {{M}olecular and phenotypic characterization of strains nodulating {A}nthyllis vulneraria in mine tailings, and proposal of {A}minobacter anthyllidis sp nov., the first definition of {A}minobacter as legume-nodulating bacteria}, author = {{M}aynaud, {G}. and {W}illems, {A}. and {S}oussou, {S}. and {V}idal, {C}. and {M}aur{\'e}, {L}. and {M}oulin, {L}ionel and {C}leyet-{M}arel, {J}. {C}. and {B}runel, {B}.}, editor = {}, language = {{ENG}}, abstract = {{B}acterial strains from {Z}n-{P}b mine tailings were isolated by trapping with {A}nthyllis vulneraria, a legume-host suitable for mine substratum phytostabilisation. {S}equence analysis of the 16{S} r{RNA} gene and three housekeeping genes (atp{D}, dna{K} and rec{A}) showed that they were related to those of the genus {A}minobacter. {DNA}-{DNA} relatedness of representative isolates supported the placement of novel strains in {A}minobacter as a new species. {P}henotypic data emphasize their differentiation from the other related species of {A}minobacter and {M}esorhizobium. {A}minobacter isolates exhibited nod{A} sequences tightly related with {M}. loti as the closest nod{A} relative. {B}y contrast, their nod{A} sequences were highly divergent from those of {M}. metallidurans, another species associated with {A}. vulneraria that carries two complete copies of nod{A}. {T}herefore, the novel bacterial strains efficient on {A}. vulneraria represented the first occurrence of legume symbionts in the genus {A}minobacter. {T}hey represent a new species for which the name {A}minobacter anthyllidis sp. nov. is proposed (type strain {STM}4645({T}) = {LMG}26462({T}) = {CFBP}7437({T})).}, keywords = {{M}esorhizobium ; {H}eavy-metal tolerance ; {M}ultilocus sequence analysis ; nod{A} ; gene ; {N}-2-fixation}, booktitle = {}, journal = {{S}ystematic and {A}pplied {M}icrobiology}, volume = {35}, numero = {2}, pages = {65--72}, ISSN = {0723-2020}, year = {2012}, DOI = {10.1016/j.syapm.2011.11.002}, URL = {https://www.documentation.ird.fr/hor/fdi:010055769}, }