%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Barnaud, Adeline
%A Vignes, H.
%A Risterucci, A. M.
%A Noyer, J. L.
%A Pham, Jean-Louis
%A Blay, Carole
%A Buiron, Marylène
%A Vigouroux, Yves
%A Billot, C.
%T Development of nuclear microsatellite markers for the fonio, digitaria exilis (poaceae), an understudied west african cereal
%D 2012
%L fdi:010055748
%G ENG
%J American Journal of Botany
%@ 0002-9122
%K Africa ; Digitaria exilis ; fonio ; genetic diversity ; Poaceae ; simple sequence repeat markers ; underutilized crops
%M ISI:000301254600007
%N 3
%P E105-E107
%R 10.3732/ajb.1100423
%U https://www.documentation.ird.fr/hor/fdi:010055748
%> https://www.documentation.ird.fr/intranet/publi/2012/04/010055748.pdf
%V 99
%W Horizon (IRD)
%X Premise of the study: We developed nuclear microsatellite primers to explore the genetic diversity, population genetic structure, and evolutionary history of the fonio (Digitaria exilis), an understudied cereal cultivated in West Africa. Methods and Results: We used a microsatellite-enriched library approach to isolate and characterize 38 nuclear primer pairs (31 di-, fi ve tri-, and two tetranucleotide repeats), of which 21 were polymorphic and exhibited a clear pattern in 36 accessions from West Africa. The number of alleles per locus ranged from two to 22, with a mean of 4.71, and expected heterozygosity ranged from 0.03 to 0.93. Conclusions: The developed set of 21 polymorphic SSR markers will provide tools for population and evolutionary genetics studies of the cultivated fonio.
%$ 076