@article{fdi:010055747,
  title = {{C}ryobanking of {K}orean allium germplasm collections : results from a 10 year experience},
  author = {{K}im, {H}.{H}. and {P}opova, {E}. and {S}hin, {D}.{J}. and {Y}i, {J}.{Y}. and {K}im, {C}.{H}. and {L}ee, {J}.{S}. and {Y}oon, {M}.{K}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his paper reviews a 10-year experience in establishing a cryopreserved {A}llium germplasm collection at the genebank of the {N}ational {A}grobiodiversity {C}enter, {R}epublic of {K}orea. {A} systematic approach to {A}llium cryopreservation included: 1. revealing the most critical factors that affected regeneration after cryostorage; 2. understanding the mechanisms of cryoprotection by analyzing the thermal behavior of explants and cryoprotectant solutions using {DSC} and influx/efflux of cryoprotectants using {HPLC}; 3. assessing genetic stability of regenerants; and 4. revealing the efficiency of cryotherapy. {B}ulbil primordia, i.e. asexual bulbs formed on unripe inflorescences, proved to be the most suitable material for conservation of bolting varieties due to high post-cryopreservation regrowth and lower microbial infection level, followed by apical shoot apices from single bulbs and cloves. {A} total of 1,158 accessions of garlic as well as some {A}llium species have been cryopreserved during 2005-2010 using the droplet-vitrification technique with a mean regeneration percentage of 65.9% after cryostorage. {T}hese results open the door for large-scale implementation of cryostorage and for simplifying international exchange for clonal {A}llium germplasm.},
  keywords = {cryopreservation ; cryotherapy ; droplet-vitrification ; garlic ; implementation},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {33},
  numero = {1},
  pages = {45--57},
  ISSN = {0143-2044},
  year = {2012},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055747},
}