@article{fdi:010055658,
  title = {{M}ultilocus sequence analysis and type {III} effector repertoire mining provide new insights into the evolutionary history and virulence of {X}anthomonas oryzae},
  author = {{H}ajri, {A}. and {B}rin, {C}. and {Z}hao, {S}. and {D}avid, {P}. and {F}eng, {J}. {X}. and {K}oebnik, {R}alf and {S}zurek, {B}oris and {V}erdier, {V}al{\'e}rie and {B}oureau, {T}. and {P}oussier, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ultilocus sequence analysis ({MLSA}) and type {III} effector ({T}3{E}) repertoire mining were performed to gain new insights into the genetic relatedness of {X}anthomonas oryzae pv. oryzae ({X}oo) and {X}anthomonas oryzae pv. oryzicola ({X}oc), two major bacterial pathogens of rice. {B}ased on a collection of 45 {A}frican and {A}sian strains, we first sequenced and analysed three housekeeping genes by {MLSA}, {B}ayesian clustering and a median-joining network approach. {S}econd, we investigated the distribution of 32 {T}3{E} genes, which are known to be major virulence factors of plant pathogenic bacteria, in all selected strains, by polymerase chain reaction and dot-blot hybridization methods. {T}he diversity observed within housekeeping genes, as well as within {T}3{E} repertoires, clearly showed that both pathogens belong to closely related, but distinct, phylogenetic groups. {I}nterestingly, these evolutionary groups are differentiated according to the geographical origin of the strains, suggesting that populations of {X}oo and {X}oc might be endemic in {A}frica and {A}sia, and thus have evolved separately. {W}e further revealed that {T}3{E} gene repertoires of both pathogens comprise core and variable gene suites that probably have distinct roles in pathogenicity and different evolutionary histories. {I}n this study, we carried out a functional analysis of xop{O}, a differential {T}3{E} gene between {X}oo and {X}oc, to determine the involvement of this gene in tissue specificity. {A}ltogether, our data contribute to a better understanding of the evolutionary history of {X}oo and {X}oc in {A}frica and {A}sia, and provide clues for functional studies aiming to understand the virulence, host and tissue specificity of both rice pathogens.},
  keywords = {{AFRIQUE} ; {ASIE}},
  booktitle = {},
  journal = {{M}olecular {P}lant {P}athology},
  volume = {13},
  numero = {3},
  pages = {288--302},
  ISSN = {1464-6722},
  year = {2012},
  DOI = {10.1111/j.1364-3703.2011.00745.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055658},
}