@article{fdi:010054729,
  title = {{E}volution in caffeoylquinic acid content and histolocalization during {C}offea canephora leaf development},
  author = {{M}ondolot, {L}. and {L}a {F}isca, {P}. and {B}uatois, {B}. and {T}alansier, {E}meline and {K}ochko, {A}lexandre de and {C}ampa, {C}laudine},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground and {A}ims {C}affeoylquinic acids are cinnamate conjugates derived from the phenylpropanoid pathway. {T}hey are generally involved in plant responses to biotic and abiotic stress and one of them, chlorogenic acid (5-{O}-caffeoylquinic acid, 5-{CQA}), is an intermediate in the lignin biosynthesis pathway. {C}affeoylquinic acids, and particularly 5-{CQA}, are accumulated in coffee beans, where they can form vacuolar complexes with caffeine. {C}offea canephora beans are known to have high caffeoylquinic acid content, but little is known about the content and diversity of these compounds in other plant parts. {T}o gain new insights into the caffeoylquinic acid metabolism of {C}. canephora, caffeoylquinic acid content and in situ localization were assessed in leaves at different growth stages. {M}ethods {HPLC} analyses of caffeoylquinic acid content of leaves was conducted in conjunction with detailed histochemical and microspectrofluorometrical analysis. {K}ey {R}esults and {C}onclusions {HPLC} analyses revealed that caffeoylquinic acid content was 10-fold lower in adult than in juvenile leaves. {T}he most abundant cinnamate conjugate was 5-{CQA}, but dicaffeoylquinic acids (particularly in juvenile leaves) and feruloylquinic acids were also present. {U}sing specific reagents, histochemical and microspectrofluorometrical analysis showed that caffeoylquinic acids (mono- and di-esters) were closely associated with chloroplasts in very young leaves. {D}uring leaf ageing, they were found to first accumulate intensively in specific chlorenchymatous bundle sheath cells and then in phloem sclerenchyma cells. {T}he association with chloroplasts suggests that caffeoylquinic acids have a protective role against light damage. {I}n older tissues, their presence in the leaf vascular system indicates that they are transported via phloem and confirms their involvement in lignification processes. {I}n accordance with the hypothesis of a complex formation with caffeine, similar tissue distribution was observed for alkaloids and this is further discussed.},
  keywords = {{C}offea canephora ; developing leaves ; caffeoylquinic acids ; alkaloids ; histolocalization ; microspectrofluorometry ; high performance liquid chromatography},
  booktitle = {},
  journal = {{A}nnals of {B}otany},
  volume = {98},
  numero = {1},
  pages = {33--40},
  ISSN = {0305-7364},
  year = {2006},
  DOI = {10.1093/aob/mcl080},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054729},
}