Qasemian L. auteur aut IRD Guiral Daniel auteur aut IRD Ziarelli F. auteur aut IRD Van Dang T. K. auteur aut IRD Farnet A. M. auteur aut IRD text journalArticle eng text/pdf born digital access Due to increasing atmospheric pollution, it has become highly important to investigate how anthropic chronic contaminations may affect ecosystem functioning. To explore the effect of polycyclic aromatic hydrocarbons (PAHs) on indigenous microbial activities, anthracene was used as a model PAH in a mesocosm experiment with Pinus halepensis litter from the Massif of Marseilleveyre (Marseille, France). The effects of anthracene on microbial activities were followed after 1- and 3- month incubations by: Catabolic Level Physiological Profile (CLPP) using ECO and FF plates and four enzyme activities (cellulase. beta-glucosidase, acid phosphatase and lipase), Moreover the chemical variations in organic matter were evaluated by solid-state C-13 NMR and C/N ratio. These experiments revealed an increase in cellulase, beta-glucosidase and phosphatase activities and a decrease in lipase activities after a 3-month incubation in the presence of anthracene. Principal Component Analysis (PCA) from CLPP showed that bacterial catabolic diversity is more influenced than that of fungal communities by anthracene. Correlation between both chemical and biological indicators revealed that the increase in lignocellulolytic enzymes (cellulase, laccase and beta-glucosidase) was significantly correlated to the decrease in phenolic compounds. In addition, aromaticity ratio also decreased in the presence of anthracene suggesting that transformation of the recalcitrant part of organic matter was enhanced. Our results highlight the difference in sensitivity of bacterial and fungal communities to PAHs, the later especially active while exposed to high concentrations of pollutant. This suggests that microbial communities inhabiting P. halepensis litters in Mediterranean coastal areas may resist to chronic pollution involving PAH. specialized Chronic pollution PAHs Lignocellulolytic activities C-13 NMR CLPP 068 084 46 148-154 2012 0038-0717 https://www.documentation.ird.fr/hor/fdi:010054420 10.1016/j.soilbio.2011.12.002 0038-0717 [F B010054420] https://www.documentation.ird.fr/hor/fdi:010054420 https://www.documentation.ird.fr/intranet/publi/2012/03/010054420.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-04-04 2017-08-23 fdi:010054420 fre