%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Jiang, Q.B.
%A Zhang, Y.
%A Zhong, C.L.
%A Zeng, B.S.
%A Bogusz, Didier
%A Franche, Claudine
%T Establishment of an in vitro plant regeneration protocol for Casuarina cunninghamiana Miq. via indirect organogenesis
%D 2012
%L fdi:010054390
%G ENG
%J New Forests
%@ 0169-4286
%K Casuarina cunninghamiana ; Micropropagation ; Indirect organogenesis ; Plant regeneration
%K ZONE TROPICALE
%M ISI:000300081900002
%N 2
%P 143-154
%R 10.1007/s11056-011-9277-5
%U https://www.documentation.ird.fr/hor/fdi:010054390
%> https://www.documentation.ird.fr/intranet/publi/2012/03/010054390.pdf
%V 43
%W Horizon (IRD)
%X An in vitro plant regeneration protocol via indirect organogenesis from morphogenetic callus was established for Casuarina cunninghamiana Miq. Effects of plant growth regulator NAA (naphthaleneacetic acid) and BAP (6-benzylaminopurine), sucrose and AgNO3 on callus induction, adventitious bud differentiation and shoot development were examined. Explants used were epicotyl fragments from 45-day-old seedlings. The largest callus (4.29 mm in diameter) was obtained after 1 month on a basic culture medium consisting of Murashige and Skoog A1/2 macro- and full strength micro- elements, Nitsch and Nitsch vitamins, supplemented with 0.54 mu M NAA, 3.30 mu M BAP, and 30 g L-1 sucrose. The calli were subcultured in the same medium above for 2 months. They were then cultured for another 2 months for adventitious bud differentiation and shoot development. The highest mean adventitious bud differentiation, number of shoots formed per callus and number of shoots a parts per thousand yen2 cm long per callus (47.50%, 27.38 and 4.75, respectively) were achieved on the above medium modified with NAA at 0.27 mu M and supplemented with AgNO3 1 mg L-1. Shoots were successfully rooted without plant growth regulator and the rooted plantlets survived and grew normally. This protocol for in vitro plant regeneration provides a tool not only for vegetative propagation but also for plant genetic transformation and gene function studies of C. cunninghamiana.
%$ 084