@article{fdi:010054334,
  title = {{A} phylogenetic strategy based on a legume-specific whole genome duplication yields symbiotic cytokinin type-a response regulators},
  author = {{O}p den {C}amp, {R}. {H}. {M}. and {D}e {M}ita, {S}t{\'e}phane and {L}illo, {A}. and {C}ao, {Q}. {Q}. and {L}impens, {E}. and {B}isseling, {T}. and {G}eurts, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}egumes host their {R}hizobium spp. symbiont in novel root organs called nodules. {N}odules originate from differentiated root cortical cells that dedifferentiate and subsequently form nodule primordia, a process controlled by cytokinin. {A} whole-genome duplication has occurred at the root of the legume {P}apilionoideae subfamily. {W}e hypothesize that gene pairs originating from this duplication event and are conserved in distinct {P}apilionoideae lineages have evolved symbiotic functions. {A} phylogenetic strategy was applied to search for such gene pairs to identify novel regulators of nodulation, using the cytokinin phosphorelay pathway as a test case. {I}n this way, two paralogous type-{A} cytokinin response regulators were identified that are involved in root nodule symbiosis. {R}esponse {R}egulator9 ({M}t{RR}9) and {M}t{RR}11 in medicago ({M}edicago truncatula) and an ortholog in lotus ({L}otus japonicus) are rapidly induced upon {R}hizobium spp. {N}od factor signaling. {C}onstitutive expression of {M}t{RR}9 results in arrested primordia that have emerged from cortical, endodermal, and pericycle cells. {I}n legumes, lateral root primordia are not exclusively formed from pericycle cells but also require the involvement of the root cortical cell layer. {T}herefore, the {M}t{RR}9-induced foci of cell divisions show a strong resemblance to lateral root primordia, suggesting an ancestral function of {M}t{RR}9 in this process. {T}ogether, these findings provide a proof of principle for the applied phylogenetic strategy to identify genes with a symbiotic function in legumes.},
  keywords = {},
  booktitle = {},
  journal = {{P}lant {P}hysiology},
  volume = {157},
  numero = {4},
  pages = {2013--2022},
  ISSN = {0032-0889},
  year = {2011},
  DOI = {10.1104/pp.111.187526},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054334},
}