Combes Marie-Christine auteur aut IRD Cenci Alberto auteur aut IRD Baraille H. auteur aut IRD Bertrand B. auteur aut IRD Lashermes Philippe auteur aut IRD text journalArticle eng text/pdf born digital access Allopolyploidy is considered as a major factor contributing to speciation, diversification, and plant ecological adaptation. In particular, the expression of duplicate genes (homeologs) can be altered leading to functional plasticity and to phenotypic novelty. This study investigated the influence of growing temperatures on homeologous gene expression in Coffea arabica L., a recent allopolyploid involving 2 closely related diploid parental species. The relative expression of homeologs of 13 genes all located in the same genomic region was analyzed using an SNP ratio quantification method based on dideoxy-terminated sequences of cDNA amplicons. The relative expression of homeologous genes varied depending on the gene, the organ, and the growing condition. Nevertheless, expression of both homeologs was always detected (i.e., no silencing). Although the growing conditions were suitable for one or other of the parental species, neither subgenome appeared preferentially expressed. Furthermore, relative homeologous expression showed moderate variations across organs and conditions and appeared uncorrelated between adjacent genes. These results indicate the absence of signs of subfunctionalization suggesting C. arabica has not undergone noticeable diploidization. Furthermore, these results suggest that the expression of homeologous genes in C. arabica is regulated by a shared trans-regulation mechanism acting similarly on the 2 subgenomes and that the observed biases in the relative homeolog expression may result from cis fine-scale factors. specialized adaptation coffee duplicate gene expression introgression polyploidy transcription 076 103 1 36-46 2012 0022-1503 https://www.documentation.ird.fr/hor/fdi:010054310 10.1093/jhered/esr120 0022-1503 [F B010054310] https://www.documentation.ird.fr/hor/fdi:010054310 https://www.documentation.ird.fr/intranet/publi/2012/01/010054310.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-02-01 2017-08-23 fdi:010054310 fre