Osorio-Saenz A. auteur aut IRD Mascorro-Gallardo J. O. auteur aut IRD Valle-Sandoval M. D. auteur aut IRD Gonzalez-Arnao M. T. auteur aut IRD Engelmann Florent auteur aut IRD text journalArticle eng text/pdf born digital access Shoot-tips isolated from two transgenic lines of chrysanthemum (Dendranthema grancliflorum Kitam.) var. Indianapolis in vitro plantlets with induced capacity to biosynthesize trehalose, and from a non-transformed line, were subjected to cryopreservation using a vitrification procedure. After dissection, apices were precultured on semi-solid MS medium with 0.3 M sucrose for 4 days, loaded in a 0.4 M sucrose + 2 M glycerol solution for 20-30 min and exposed to PVS2 or PVS3 vitrification solutions for 0, 20, 40 or 60 min at room temperature prior to rapid immersion in liquid nitrogen. The highest shoot regeneration after cryopreservation was obtained with exposure to either PVS solution for 40 min. Plant regeneration from cryopreserved shoot-tips ranged between 48% and 67% for transgenic lines and between 33% and 36% for non-transgenic lines. No polymorphic loci were detected in plantlets regenerated from cryopreserved and non-cryopreserved shoot-tips with RAPD techniques using eight primers that amplified 101 monomorphic loci. specialized chrysanthemum trehalose transgenic plantlets shoot-tips vitrification 076 084 32 6 477-486 2011 0143-2044 https://www.documentation.ird.fr/hor/fdi:010054287 0143-2044 [F B010054287] https://www.documentation.ird.fr/hor/fdi:010054287 https://www.documentation.ird.fr/intranet/publi/2012/01/010054287.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-02-01 2017-08-23 fdi:010054287 fre