@article{fdi:010054287,
  title = {{G}eneticallly enginereed trehalose accumulation improves cryopreservation tolerance of chrysanthemum ({D}endranthema grandiflorum {K}itam) shoot-tips},
  author = {{O}sorio-{S}aenz, {A}. and {M}ascorro-{G}allardo, {J}. {O}. and {V}alle-{S}andoval, {M}. {D}. and {G}onzalez-{A}rnao, {M}. {T}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{S}hoot-tips isolated from two transgenic lines of chrysanthemum ({D}endranthema grancliflorum {K}itam.) var. {I}ndianapolis in vitro plantlets with induced capacity to biosynthesize trehalose, and from a non-transformed line, were subjected to cryopreservation using a vitrification procedure. {A}fter dissection, apices were precultured on semi-solid {MS} medium with 0.3 {M} sucrose for 4 days, loaded in a 0.4 {M} sucrose + 2 {M} glycerol solution for 20-30 min and exposed to {PVS}2 or {PVS}3 vitrification solutions for 0, 20, 40 or 60 min at room temperature prior to rapid immersion in liquid nitrogen. {T}he highest shoot regeneration after cryopreservation was obtained with exposure to either {PVS} solution for 40 min. {P}lant regeneration from cryopreserved shoot-tips ranged between 48% and 67% for transgenic lines and between 33% and 36% for non-transgenic lines. {N}o polymorphic loci were detected in plantlets regenerated from cryopreserved and non-cryopreserved shoot-tips with {RAPD} techniques using eight primers that amplified 101 monomorphic loci.},
  keywords = {chrysanthemum ; trehalose ; transgenic plantlets ; shoot-tips ; vitrification},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {32},
  numero = {6},
  pages = {477--486},
  ISSN = {0143-2044},
  year = {2011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054287},
}