@article{fdi:010054286,
  title = {{I}mproved cryopreservation of chrysanthemum ({C}hrysanthemum morifolium) using droplet-vitrification},
  author = {{L}ee, {Y}. {G}. and {P}opova, {E}. and {C}ui, {H}. {Y}. and {K}im, {H}. {H}. and {P}ark, {S}. {U}. and {B}ae, {C}. {H}. and {L}ee, {S}. {C}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A} droplet-vitrification protocol has been established for cryopreserving {C}hrysanthemum morifolium cv. {P}eak using axillary shoot tips and apical shoots of in vitro plants. {I}n the optimized procedure, explants were submitted to a step-wise preculture in liquid sucrose-enriched medium (0.3, 0.5 and 0.7 {M} for 31,17 and 7 h, respectively). {P}recultured explants were treated for 40 min with {C}4 loading solution comprising (w/v) 17.5% glycerol + 17.5% sucrose, then dehydrated with {PVS}3 vitrification solution (w/v, 50% glycerol + 50% sucrose) for 60 min (axillary shoot tips) or 90 min (apical shoots). {E}xplants were cryopreserved by direct immersion in liquid nitrogen in minute drops of {PVS}3 attached to aluminum foil strips. {T}he optimal age of donor plants was 4-5.5 weeks for apical shoots and 7 weeks for axillary shoot tips, producing post-cryopreservation regeneration percentages of 81.9% and 84.9%, respectively. {P}lants regenerated from cryopreserved samples showed no phenotypical abnormalities and similar profiles of relative {DNA} content were recorded for control and cryopreserved plants. {O}ur results suggest that the modified droplet-vitrification protocol described in this paper is highly effective and may prove user-friendlier than the cryopreservation protocols already published for chrysanthemum.},
  keywords = {loading ; preculture ; relative {DNA} content ; shoot tips ; vitrification solution},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {32},
  numero = {6},
  pages = {487--497},
  ISSN = {0143-2044},
  year = {2011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054286},
}