@article{fdi:010054279,
  title = {454-pyrosequencing of {C}offea arabica leaves infected by the rust fungus {H}emileia vastatrix reveals in planta-expressed pathogen-secreted proteins and plant functions in a late compatible plant-rust interaction},
  author = {{F}ernandez, {D}iana and {T}isserant, {E}. and {T}alhinhas, {P}. and {A}zinheira, {H}. and {V}ieira, {A}. and {P}etitot, {A}nne-{S}ophie and {L}oureiro, {A}. and {P}oulain, {J}. and {D}a {S}ilva, {C}. and {S}ilva, {M}. {D}. {C}. and {D}uplessis, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}offee ({C}offea arabica {L}.), one of the key export and cash crops in tropical and subtropical countries, suffers severe losses from the rust fungus {H}emileia vastatrix. {T}he transcriptome of {H}. vastatrix was analysed during a compatible interaction with coffee to obtain an exhaustive repertoire of the genes expressed during infection and to identify potential effector genes. {L}arge-scale sequencing (454-{GS}-{FLEX} {T}itanium) of mixed coffee and rust c{DNA}s obtained from 21-day rust-infected leaves generated 352 146 sequences which assembled into 22 774 contigs. {I}n the absence of any reference genomic sequences for {C}offea or {H}emileia, specific trinucleotide frequencies within expressed sequence tags ({EST}s) and blast homology against a set of dicots and basidiomycete genomes were used to distinguish pathogen from plant sequences. {A}bout 30% (6763) of the contigs were assigned to {H}. vastatrix and 61% (13 951) to {C}. arabica. {T}he majority (60%) of the rust sequences did not show homology to any genomic database, indicating that they were potential novel fungal genes. {I}n silico analyses of the 6763 {H}. vastatrix contigs predicted 382 secreted proteins and identified homologues of the flax rust haustorially expressed secreted proteins ({HESP}s) and bean rust transferred protein 1 ({RTP}1). {T}hese rust candidate effectors showed conserved amino-acid domains and conserved patterns of cysteine positions suggestive of conserved functions during infection of host plants. {Q}uantitative reverse transcription-polymerase chain reaction profiling of selected rust genes revealed dynamic expression patterns during the time course of infection of coffee leaves. {T}his study provides the first valuable genomic resource for the agriculturally important plant pathogen {H}. vastatrix and the first comprehensive {C}. arabica {EST} dataset.},
  keywords = {},
  booktitle = {},
  journal = {{M}olecular {P}lant {P}athology},
  volume = {13},
  numero = {1},
  pages = {17--37},
  ISSN = {1464-6722},
  year = {2012},
  DOI = {10.1111/j.1364-3703.2011.00723.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054279},
}