Talamond Pascale auteur aut IRD Conejero G. auteur aut IRD Verdeil J.L. auteur aut IRD Poessel J.L. auteur aut IRD text journalArticle eng text/pdf born digital access The biochemical composition of leaves from Coffea pseudozanguebariae, a wild caffeine-free coffee species, was determined. Two phenolic compounds were extracted from leaves, separated and characterized. Their structures were elucidated by mass spectrometry, and 1D and 2D NMR spectroscopy and were shown to be mangiferin (1) and isomangiferin (2), which were the main polyphenol products. Multiphoton fluorescence imaging was performed to visualize polyphenol distribution in leaf cross sections. Consistent biochemical analysis cell imaging techniques on leaves revealed yellow fluorescence in the epidermis and parenchyma cells corresponding to xanthone compounds. specialized Coffea pseudozanguebariae Bridson xanthones fluorescence mangiferin isomangiferin Rubiaceae 076 020 6 12 1885-1888 2011 1934-578X https://www.documentation.ird.fr/hor/fdi:010054269 1934-578X [F B010054269] https://www.documentation.ird.fr/hor/fdi:010054269 https://www.documentation.ird.fr/intranet/publi/2012/01/010054269.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-02-01 2017-08-23 fdi:010054269 fre