@article{fdi:010054203,
  title = {{I}mproving the population genetics toolbox for the study of the {A}frican malaria vector {A}nopheles nili : microsatellite mapping to chromosomes},
  author = {{P}eery, {A}. and {S}harakhova, {M}. {V}. and {A}ntonio-{N}kondjio, {C}. and {N}do, {C}. and {W}eill, {M}. and {S}imard, {F}r{\'e}d{\'e}ric and {S}harakhov, {I}. {V}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {A}nopheles nili is a major vector of malaria in the humid savannas and forested areas of sub-{S}aharan {A}frica. {U}nderstanding the population genetic structure and evolutionary dynamics of this species is important for the development of an adequate and targeted malaria control strategy in {A}frica. {C}hromosomal inversions and microsatellite markers are commonly used for studying the population structure of malaria mosquitoes. {P}hysical mapping of these markers onto the chromosomes further improves the toolbox, and allows inference on the demographic and evolutionary history of the target species. {R}esults: {A}vailability of polytene chromosomes allowed us to develop a map of microsatellite markers and to study polymorphism of chromosomal inversions. {N}ine microsatellite markers were mapped to unique locations on all five chromosomal arms of {A}n. nili using fluorescent in situ hybridization ({FISH}). {P}robes were obtained from 300-483 bp-long inserts of plasmid clones and from 506-559 bp-long fragments amplified with primers designed using the {A}n. nili genome assembly generated on an {I}llumina platform. {T}wo additional loci were assigned to specific chromosome arms of {A}n. nili based on in silico sequence similarity and chromosome synteny with {A}nopheles gambiae. {T}hree microsatellites were mapped inside or in the vicinity of the polymorphic chromosomal inversions 2{R}b and 2{R}c. {A} statistically significant departure from {H}ardy-{W}einberg equilibrium, due to a deficit in heterozygotes at the 2{R}b inversion, and highly significant linkage disequilibrium between the two inversions, were detected in natural {A}n. nili populations collected from {B}urkina {F}aso. {C}onclusions: {O}ur study demonstrated that next-generation sequencing can be used to improve {FISH} for microsatellite mapping in species with no reference genome sequence. {P}hysical mapping of microsatellite markers in {A}n. nili showed that their cytological locations spanned the entire five-arm complement, allowing genome-wide inferences. {T}he knowledge about polymorphic inversions and chromosomal locations of microsatellite markers has been useful for explaining differences in genetic variability across loci and significant differentiation observed among natural populations of {A}n. nili.},
  keywords = {{C}hromosome inversions ; genome sequence ; malaria vector ; microsatellite ; markers ; population structure},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {4},
  numero = {},
  pages = {202},
  ISSN = {1756-3305},
  year = {2011},
  DOI = {10.1186/1756-3305-4-202},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054203},
}