@article{fdi:010054201,
  title = {{D}engue virus replication in infected human keratinocytes leads to activation of antiviral innate immune responses},
  author = {{S}urasombatpattana, {P}ornapat and {H}amel, {R}odolphe and {P}atramool, {S}. and {L}uplertlop, {N}. and {T}homas, {F}. and {D}espres, {P}. and {B}riant, {L}. and {Y}ssel, {H}. and {M}iss{\'e}, {D}oroth{\'e}e},
  editor = {},
  language = {{ENG}},
  abstract = {{D}engue virus ({DENV}) infection is the most prevalent mosquito-borne viral diseases in the world. {V}ector-mediated transmission of {DENV} is initiated when a blood-feeding female {A}edes mosquito injects saliva, together with the virus, into the skin of its mammalian host. {U}nderstanding the role of skin immune cells in the activation of innate immunity to {DENV} at the early times of infection is a critical issue that remains to be investigated. {T}he purpose of our study was to assess the contribution of human keratinocytes as potential host cells to {DENV} in the activation of immune responses at the anatomical site of mosquito bite. {W}e show that primary keratinocytes support {DENV} replication with the production of negative-stranded viral {RNA}s inside the infected cells. {I}n the course of {DENV} life cycle, we observed the activation of host genes involved in the antiviral immune responses such as intracellular {RNA} virus sensors {T}oll-{L}ike {R}eceptor-3, {R}etinoic {A}cid {I}nducible {G}ene-{I}, {M}elanoma {D}ifferentiation {A}ssociated gene-5 and the {RNA}-dependent protein kinase {R}. {DENV} infection of primary keratinocytes also resulted in up-regulation of the expression of the antiviral {R}ibonuclease {L} gene, which subsequently led to enhanced production of {IFN}-beta and {IFN}-gamma. {D}epending on stages of viral replication, we observed the activation of host genes encoding the antimicrobial proteins beta-defensin and {RN}ase 7 in infected keratinocytes. {O}ur data demonstrate for the first time the permissiveness of human epidermal keratinocytes to {DENV} infection. {R}emarkably, {DENV} replication in keratinocytes contributes to the establishment of antiviral innate immunity that might occur in the early times after the bite of mosquito.},
  keywords = {{D}engue virus ; {K}eratinocyte ; {I}nnate immunity ; {I}nterferon ; {RN}ase {L} ; {RIG}-{I}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {11},
  numero = {7},
  pages = {1664--1673},
  ISSN = {1567-1348},
  year = {2011},
  DOI = {10.1016/j.meegid.2011.06.009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054201},
}