%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Barnabé, Christian
%A De Meeûs, Thierry
%A Noireau, François
%A Bosseno, Marie-France
%A Monje, E. M.
%A Renaud, François
%A Brenière, Simone Frédérique
%T Trypanosoma cruzi discrete typing units (DTUs) : microsatellite loci and population genetics of DTUs TcV and TcI in Bolivia and Peru
%D 2011
%L fdi:010054199
%G ENG
%J Infection Genetics and Evolution
%@ 1567-1348
%K Trypanosoma cruzi ; Multilocus microsatellite typing ; Discrete typing ; units ; Populations ; Genetics ; Reproductive systems
%M ISI:000297126800029
%N 7
%P 1752-1760
%R 10.1016/j.meegid.2011.07.011
%U https://www.documentation.ird.fr/hor/fdi:010054199
%> https://www.documentation.ird.fr/intranet/publi/2011/12/010054199.pdf
%V 11
%W Horizon (IRD)
%X Trypanosoma cruzi, the agent of Chagas disease, is usually subdivided into six discrete typing units (DTUs), TcI to TcVI, among which TcI and TcV are most common in human infections in Bolivia. Multilocus microsatellite typing (MLMT) was selected to further explore the structure of the natural populations belonging to these DTUs. The analysis showed that microsatellite clustering does not fully match the six DTUs, but it is relevant for the within DTUs analyses. Population genetics analysis was conducted on 11 relevant subsamples of stocks from Bolivia and Peru, belonging to TcI (6) and TcV (5), defined by four criterions: DTU, vector species, geographic origin, and date of isolation. Most TcV strains presented the same multilocus genotype over all subsamples with the puzzling characteristic that five loci were heterozygous and the other five homozygous. In TcI, four clusters were defined according to the vector species. Most of them appeared in agreement with clonal propagation (stocks isolated from Triatoma infestans and Triatoma sordida), while a few highly homozygous stocks (e.g. those isolated from Rhodnius stali) suggested that scarce sex events can occur. The poor role played by spatio-temporal factors in describing the observed genetic diversity suggested that ecology, in particular as regard to host played a significant role. These results highlight the extreme heterogeneity of T. cruzi and suggest that further population genetics surveys will need to target the most possible precise spatio-temporal and ecological scales.
%$ 052