@article{fdi:010054199,
  title = {{T}rypanosoma cruzi discrete typing units ({DTU}s) : microsatellite loci and population genetics of {DTU}s {T}c{V} and {T}c{I} in {B}olivia and {P}eru},
  author = {{B}arnab{\'e}, {C}hristian and {D}e {M}eeûs, {T}hierry and {N}oireau, {F}ran{\c{c}}ois and {B}osseno, {M}arie-{F}rance and {M}onje, {E}. {M}. and {R}enaud, {F}ran{\c{c}}ois and {B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique},
  editor = {},
  language = {{ENG}},
  abstract = {{T}rypanosoma cruzi, the agent of {C}hagas disease, is usually subdivided into six discrete typing units ({DTU}s), {T}c{I} to {T}c{VI}, among which {T}c{I} and {T}c{V} are most common in human infections in {B}olivia. {M}ultilocus microsatellite typing ({MLMT}) was selected to further explore the structure of the natural populations belonging to these {DTU}s. {T}he analysis showed that microsatellite clustering does not fully match the six {DTU}s, but it is relevant for the within {DTU}s analyses. {P}opulation genetics analysis was conducted on 11 relevant subsamples of stocks from {B}olivia and {P}eru, belonging to {T}c{I} (6) and {T}c{V} (5), defined by four criterions: {DTU}, vector species, geographic origin, and date of isolation. {M}ost {T}c{V} strains presented the same multilocus genotype over all subsamples with the puzzling characteristic that five loci were heterozygous and the other five homozygous. {I}n {T}c{I}, four clusters were defined according to the vector species. {M}ost of them appeared in agreement with clonal propagation (stocks isolated from {T}riatoma infestans and {T}riatoma sordida), while a few highly homozygous stocks (e.g. those isolated from {R}hodnius stali) suggested that scarce sex events can occur. {T}he poor role played by spatio-temporal factors in describing the observed genetic diversity suggested that ecology, in particular as regard to host played a significant role. {T}hese results highlight the extreme heterogeneity of {T}. cruzi and suggest that further population genetics surveys will need to target the most possible precise spatio-temporal and ecological scales.},
  keywords = {{T}rypanosoma cruzi ; {M}ultilocus microsatellite typing ; {D}iscrete typing ; units ; {P}opulations ; {G}enetics ; {R}eproductive systems},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {11},
  numero = {7},
  pages = {1752--1760},
  ISSN = {1567-1348},
  year = {2011},
  DOI = {10.1016/j.meegid.2011.07.011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054199},
}