@article{fdi:010054186,
  title = {{P}hylogenetic utility of the nuclear genes {AGAMOUS} 1 and {PHYTOCHROME} {B} in palms ({A}recaceae) : an example within {B}actridinae},
  author = {{L}udena, {B}. and {C}habrillange, {N}athalie and {A}berlenc {B}ertossi, {F}r{\'e}d{\'e}rique and {A}dam, {H}{\'e}l{\`e}ne and {T}regear, {J}ames and {P}intaud, {J}ean-{C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground and {A}ims {M}olecular phylogenetic studies of palms ({A}recaceae) have not yet provided a fully resolved phylogeny of the family. {T}here is a need to increase the current set of markers to resolve difficult groups such as the {N}eotropical subtribe {B}actridinae ({A}recoideae: {C}ocoseae). {W}e propose the use of two single-copy nuclear genes as valuable tools for palm phylogenetics. {M}ethods {N}ew primers were developed for the amplification of the {AGAMOUS} 1 ({AG}1) and {PHYTOCHROME} {B} ({PHYB}) genes. {F}or the {AGAMOUS} gene, the paralogue 1 of {E}laeis guineensis ({E}g{AG}1) was targeted. {T}he region amplified contained coding sequences between the {MIKC} {K} and {C} {MADS}-box domains. {F}or the {PHYB} gene, exon 1 (partial sequence) was first amplified in palm species using published degenerate primers for {P}oaceae, and then specific palm primers were designed. {T}he two gene portions were sequenced in 22 species of palms representing all genera of {B}actridinae, with emphasis on {A}strocaryum and {H}exopetion, the status of the latter genus still being debated. {K}ey {R}esults {T}he new primers designed allow consistent amplification and high-quality sequencing within the palm family. {T}he two loci studied produced more variability than chloroplast loci and equally or less variability than {PRK}, {RPBII} and {ITS} nuclear markers. {T}he phylogenetic structure obtained with {AG}1 and {PHYB} genes provides new insights into intergeneric relationships within the {B}actridinae and the intrageneric structure of {A}strocaryum. {T}he {H}exopetion clade was recovered as monophyletic with both markers and was weakly supported as sister to {A}strocaryum sensu stricto in the combined analysis. {T}he rare {A}strocaryum minus formed a species complex with {A}strocaryum gynacanthum. {M}oreover, both {AG}1 and {PHYB} contain a microsatellite that could have further uses in species delimitation and population genetics. {C}onclusions {AG}1 and {PHYB} provide additional phylogenetic information within the palm family, and should prove useful in combination with other genes to improve the resolution of palm phylogenies.},
  keywords = {{AGAMOUS} 1 ; {PHYTOCHROME} {B} ; phylogenetic markers ; microsatellite ; palms ; {A}recaceae ; {B}actridinae ; {A}crocomia ; {A}iphanes ; {A}strocaryum ; {B}actris ; {D}esmoncus ; {H}exopetion},
  booktitle = {},
  journal = {{A}nnals of {B}otany},
  volume = {108},
  numero = {8 : {S}pecial {I}ssue : {P}alm {B}iology},
  pages = {1433--1444},
  ISSN = {0305-7364},
  year = {2011},
  DOI = {10.1093/aob/mcr191},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054186},
}