@article{fdi:010054152,
  title = {{I}dentification and characterization of the {N}on-race specific {D}isease {R}esistance 1 ({NDR}1) orthologous protein in coffee},
  author = {{C}acas, {J}. {L}. and {P}etitot, {A}nne-{S}ophie and {B}ernier, {L}. and {E}stevan, {J}. and {C}onejero, {G}. and {M}ongrand, {S}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {L}eaf rust, which is caused by the fungus {H}emileia vastatrix ({P}ucciniales), is a devastating disease that affects coffee plants ({C}offea arabica {L}.). {D}isadvantages that are associated with currently developed phytoprotection approaches have recently led to the search for alternative strategies. {T}hese include genetic manipulations that constitutively activate disease resistance signaling pathways. {H}owever, molecular actors of such pathways still remain unknown in {C}. arabica. {I}n this study, we have isolated and characterized the coffee {NDR}1 gene, whose {A}rabidopsis ortholog is a well-known master regulator of the hypersensitive response that is dependent on coiled-coil type {R}-proteins. {R}esults: {T}wo highly homologous c{DNA}s coding for putative {NDR}1 proteins were identified and cloned from leaves of coffee plants. {O}ne of the candidate coding sequences was then expressed in the {A}rabidopsis knock-out null mutant ndr1-1. {U}pon a challenge with a specific strain of the bacterium {P}seudomonas syringae ({DC}3000::{A}vr{R}pt2), analysis of both macroscopic symptoms and in planta microbial growth showed that the coffee c{DNA} was able to restore the resistance phenotype in the mutant genetic background. {T}hus, the c{DNA} was dubbed {C}a{NDR}1a (standing for {C}offea arabica {N}on-race specific {D}isease {R}esistance 1a). {F}inally, biochemical and microscopy data were obtained that strongly suggest the mechanistic conservation of the {NDR}1-driven function within coffee and {A}rabidopsis plants. {U}sing a transient expression system, it was indeed shown that the {C}a{NDR}1a protein, like its {A}rabidopsis counterpart, is localized to the plasma membrane, where it is possibly tethered by means of a {GPI} anchor. {C}onclusions: {O}ur data provide molecular and genetic evidence for the identification of a novel functional {NDR}1 homolog in plants. {A}s a key regulator initiating hypersensitive signalling pathways, {C}a{NDR}1 gene(s) might be target (s) of choice for manipulating the coffee innate immune system and achieving broad spectrum resistance to pathogens. {G}iven the potential conservation of {NDR}1-dependent defense mechanisms between {A}rabidopsis and coffee plants, our work also suggests new ways to isolate the as-yet-unidentified {R}-gene(s) responsible for resistance to {H}. vastatrix.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {P}lant {B}iology},
  volume = {11},
  numero = {},
  pages = {144},
  ISSN = {1471-2229},
  year = {2011},
  DOI = {10.1186/1471-2229-11-144},
  URL = {https://www.documentation.ird.fr/hor/fdi:010054152},
}