Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Journal of Infectious Diseases S800-S803 2011 fdi:010053909 ENG Journal of Infectious Diseases 0022-1899 ISI:000295991400008 Suppl. 3 10.1093/infdis/jir358 https://www.documentation.ird.fr/hor/fdi:010053909 https://www.documentation.ird.fr/intranet/publi/2011/11/010053909.pdf 204 Horizon (IRD) Marburg virus (MARV) nucleic acid was detected in Rousettus aegyptiacus bats in 2005 and 2006 in the midwest and southeast of Gabon. In this study we used MARV-specific real-time reverse-transcription polyermase chain reaction (RT-PCR) and MARV-specific nested RT-PCR assay to screen 1257 bats caught during July 2009, December 2009, and June 2010 in 3 caves situated in northern Gabon. Nine specimens tested positive by the real-time assay, with cycle threshold values ranging from 35 to 39, of which only 1 R. aegyptiacus specimen collected in 2009 was positive in the nested VP35 RT-PCR assay. Together with MARV-positive bats in the south and west found in 2005 and 2006, confirmation of phylogenetically closely related MARV-positive bats 5 years later and in northern Gabon suggests that MARV is now enzootic in Gabon and emphasizes the importance of long-term monitoring of bat populations and human-bat interfaces. 052 UR224 Gabon