Imanishi L. auteur aut IRD Vayssières Alice auteur aut IRD Franche Claudine auteur aut IRD Bogusz Didier auteur aut IRD Wall L. auteur aut IRD Svistoonoff Sergio auteur aut IRD text journalArticle eng text/pdf born digital access Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia bacteria, we set up an efficient genetic transformation protocol for D. trinervis based on Agrobacterium rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11::GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses. specialized 076 084 24 11 1317-1324 2011 0894-0282 https://www.documentation.ird.fr/hor/fdi:010053905 10.1094/mpmi-03-11-0078 0894-0282 [F B010053905] https://www.documentation.ird.fr/hor/fdi:010053905 https://www.documentation.ird.fr/intranet/publi/2011/11/010053905.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-11-30 2024-04-29 fdi:010053905 fre