@article{fdi:010053905,
  title = {{T}ransformed hairy roots of {D}iscaria trinervis : valuable tool for studying actinorhizal symbiosis in the context of intercellular infection},
  author = {{I}manishi, {L}. and {V}ayssi{\`e}res, {A}lice and {F}ranche, {C}laudine and {B}ogusz, {D}idier and {W}all, {L}. and {S}vistoonoff, {S}ergio},
  editor = {},
  language = {{ENG}},
  abstract = {{A}mong infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. {I}ntercellular infection has been described in {D}iscaria trinervis, an actinorhizal plant belonging to the {R}osales order. {T}o decipher the molecular mechanisms underlying intercellular infection with {F}rankia bacteria, we set up an efficient genetic transformation protocol for {D}. trinervis based on {A}grobacterium rhizogenes. {W}e showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by {F}rankia strain {BCU}110501. {N}itrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. {I}n order to challenge the transformation system, the {M}t{E}nod11 promoter, a gene from {M}edicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in {D}. trinervis. {M}t{E}nod11::{GUS} expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. {T}he ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses.},
  keywords = {},
  booktitle = {},
  journal = {{M}olecular {P}lant-{M}icrobe {I}nteractions},
  volume = {24},
  numero = {11},
  pages = {1317--1324},
  ISSN = {0894-0282},
  year = {2011},
  DOI = {10.1094/mpmi-03-11-0078},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053905},
}