Le Goff Gilbert auteur aut IRD Revollo J. auteur aut IRD Guerra M. auteur aut IRD Cruz M. auteur aut IRD Simon Z. B. auteur aut IRD Roca Y. auteur aut IRD Flores J.V. auteur aut IRD Hervé Jean-Pierre auteur aut IRD text journalArticle eng text/pdf reformatted digital access The natural transmission of dengue virus from an infected female mosquito to its progeny, namely the vertical transmission, was researched in wild caught Aedes aegypti during an important outbreak in the town of Santa Cruz de la Sierra, Bolivia. Mosquitoes were collected at the preimaginal stages (eggs, larvae and pupae) then reared up to adult stage for viral detection using molecular methods. Dengue virus serotypes 1 and 3 were found to be co-circulating with significant higher prevalence in male than in female mosquitoes. Of the 97 pools of Ae. aegypti (n = 635 male and 748 female specimens) screened, 14 pools, collected in February-May in 2007, were found positive for dengue virus infection: five DEN-1 and nine DEN-3. The average true infection rate (TIR) and minimum infection rate (MIR) were respectively 1.08 % and 1.01 %. These observations suggest that vertical transmission of dengue virus may be detected in vectors at the peak of an outbreak as well as several months before an epidemic occurs in human population. specialized Aedes aegypti vertical transmission dengue virus PCR BOLIVIE 052 18 3 277-280 2011 1252-607X https://www.documentation.ird.fr/hor/fdi:010053896 1252-607X [F B010053896] https://www.documentation.ird.fr/hor/fdi:010053896 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010053896.pdf IRD - Base Horizon / Pleins textes 2011-11-07 2021-10-01 fdi:010053896 fre