@article{fdi:010053864,
  title = {{G}razing by nematodes on rhizosphere bacteria enhances nitrate and phosphorus availability to {P}inus pinaster seedlings},
  author = {{I}rshad, {U}. and {V}illenave, {C}{\'e}cile and {B}rauman, {A}lain and {P}lassard, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he microbial loop is thought to play a major role in the mineralization of nutrients such as nitrogen ({N}) and phosphorus ({P}) in terrestrial ecosystems. {T}his microbial loop is based on the grazing of bacteria by predators such as bacterial-feeding nematodes. {H}owever, little is known about the impact of grazing by nematodes on the mineral nutrition of woody plants. {T}his study was undertaken to quantify the effect of nematode grazing on bacteria in the rhizosphere on the root architecture, growth and mineral nutrition ({N} and {P}) of a woody species ({P}inus pinaster). {Y}oung {P}. pinaster seedlings were cultivated for 35 days in a simplified sterile experimental system with bacteria ({B}acillus subtilis) and bacterivorous nematodes ({R}habditis sp.) isolated from soil samples collected from a 15-year old stand of maritime pine. {T}o check the hypothesis that bacteria could be a source of nutrients, especially {N}, two {N} sources were supplied in the medium: (i) bacterial {N} labeled with {N}-15 and (ii) nitrate. {P}hosphorus was supplied as insoluble inorganic tri-calcium phosphate ({TCP}). {T}he results showed that the {N}-15 flow from the bacteria to the plant shoots was only significant when nematodes were present, with an average accumulation of 14 +/- 5 mu g plant(-1) of {N}-15. {P}lants cultivated with nematodes also accumulated significantly more total {N} in their shoots than sterile ones or inoculated with bacteria, resulting in a net average increase in {N} of 700 mu g plant(-1). {T}he same result was observed for the total {P} accumulation in the shoots, as plants with nematodes accumulated an average of 300 mu g plant(-1) more {P} than sterile ones or inoculated with bacteria. {H}owever, the presence of bacteria, whether alone or with nematodes, did not modify the root architecture. {T}hese results demonstrated that the presence of bacterial-feeding nematodes significantly enhanced {N} and {P} availability to {P}. pinaster seedlings, probably by improving plant use of nitrate and insoluble {P} supplied in the medium.},
  keywords = {{T}rophic relationship ; {M}ineral nutrition ; {W}oody plant ; {I}norganic {P} ; {S}oil bacteria ; {B}acterial-feeding nematodes ; {R}habditis sp. ; {B}acillus subtilis},
  booktitle = {},
  journal = {{S}oil {B}iology and {B}iochemistry},
  volume = {43},
  numero = {10},
  pages = {2121--2126},
  ISSN = {0038-0717},
  year = {2011},
  DOI = {10.1016/j.soilbio.2011.06.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053864},
}