@article{fdi:010053847,
  title = {{V}ariations of {DNA} methylation in {E}ucalyptus urophylla x {E}ucalyptus grandis shoot tips and apical meristems of different physiological ages},
  author = {{M}ankessi, {F}. and {S}aya, {A}. {R}. and {F}avreau, {B}. and {D}oulbeau, {S}ylvie and {C}onejero, {G}. and {L}artaud, {M}. and {V}erdeil, {J}. {L}. and {M}onteuuis, {O}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}lobal {DNA} methylation was assessed by high-performance liquid chromatography ({HPLC}) for the first time in {E}ucalyptus urophylla x {E}ucalyptus grandis shoot tips comparing three outdoor and one in vitro sources of related genotypes differing in their physiological age. {T}he {DNA} methylation levels found were consistent with those reported for other {A}ngiosperms using the same {HPLC} technology. {N}otwithstanding noticeable time-related fluctuations within each source of plant material, methylation rate was overall higher for the mature clone (13.7%) than for the rejuvenated line of the same clone (12.6%) and for the juvenile offspring seedlings (11.8%). {T}he in vitro microshoots of the mature clone were less methylated (11.3%) than the other outdoor origins, but the difference with the juvenile seedlings was not significant. {I}mmunofluorescence investigations on shoot apices established that the mature source could be distinguished from the rejuvenated and juvenile origins by a higher density of cells with methylated nuclei in leaf primordia. {S}hoot apical meristems ({SAM}s) from the mature clone also showed a greater proportion and more methylated cells than {SAM}s from the rejuvenated and juvenile origins. {T}he nuclei of these latter were characterized by fewer and more dispersed labeled spots than for the mature source. {O}ur findings establish that physiological ageing induced quantitative and qualitative variations of {DNA} methylation at shoot tip, {SAM} and even cellular levels. {O}verall this {DNA} methylation increased with maturation and conversely decreased with rejuvenation to reach the lower scores and to show the immunolabeling patterns that characterized juvenile material nuclei.},
  keywords = {},
  booktitle = {},
  journal = {{P}hysiologia {P}lantarum},
  volume = {143},
  numero = {2},
  pages = {178--187},
  ISSN = {0031-9317},
  year = {2011},
  DOI = {10.1111/j.1399-3054.2011.01491.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053847},
}