Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Scientia Horticulturae 320-324 Sugarcane Cryopreservation Encapsulation-dehydration Droplet-vitrification 2011 fdi:010053842 ENG Scientia Horticulturae 0304-4238 ISI:000294942900047 1 10.1016/j.scienta.2011.07.003 https://www.documentation.ird.fr/hor/fdi:010053842 https://www.documentation.ird.fr/intranet/publi/2011/10/010053842.pdf 130 Horizon (IRD) In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33-37% for clone H70-144 and 20-27% for clone CP68-1026. Optimal conditions included preculture of encapsulated shoot apices for 2411 in liquid medium with 0.75 M sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. With both protocols employed, regrowth of cryopreserved samples, as followed by visual observation, was always rapid and direct. 076 ; 084 UR232