%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Barraco, Giuseppe
%A Sylvestre, Isabelle
%A Engelmann, Florent
%T Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips
%D 2011
%L fdi:010053842
%G ENG
%J Scientia Horticulturae
%@ 0304-4238
%K Sugarcane ; Cryopreservation ; Encapsulation-dehydration ; Droplet-vitrification
%M ISI:000294942900047
%N 1
%P 320-324
%R 10.1016/j.scienta.2011.07.003
%U https://www.documentation.ird.fr/hor/fdi:010053842
%> https://www.documentation.ird.fr/intranet/publi/2011/10/010053842.pdf
%V 130
%W Horizon (IRD)
%X In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33-37% for clone H70-144 and 20-27% for clone CP68-1026. Optimal conditions included preculture of encapsulated shoot apices for 2411 in liquid medium with 0.75 M sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. With both protocols employed, regrowth of cryopreserved samples, as followed by visual observation, was always rapid and direct.
%$ 076 ; 084