<?xml version="1.0"?>
<oai_dc:dc xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:title>Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips</dc:title>
  <dc:creator>Barraco, Giuseppe</dc:creator>
  <dc:creator>/Sylvestre, Isabelle</dc:creator>
  <dc:creator>/Engelmann, Florent</dc:creator>
  <dc:subject>Sugarcane</dc:subject>
  <dc:subject>Cryopreservation</dc:subject>
  <dc:subject>Encapsulation-dehydration</dc:subject>
  <dc:subject>Droplet-vitrification</dc:subject>
  <dc:description>In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33-37% for clone H70-144 and 20-27% for clone CP68-1026. Optimal conditions included preculture of encapsulated shoot apices for 2411 in liquid medium with 0.75 M sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. With both protocols employed, regrowth of cryopreserved samples, as followed by visual observation, was always rapid and direct.</dc:description>
  <dc:date>2011</dc:date>
  <dc:type>text</dc:type>
  <dc:identifier>https://www.documentation.ird.fr/hor/fdi:010053842</dc:identifier>
  <dc:identifier>fdi:010053842</dc:identifier>
  <dc:identifier>Barraco Giuseppe, Sylvestre Isabelle, Engelmann Florent. Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips. 2011, 130 (1),  320-324</dc:identifier>
  <dc:language>EN</dc:language>
</oai_dc:dc>