@article{fdi:010053842,
  title = {{C}omparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane ({S}accharum spp.) shoot tips},
  author = {{B}arraco, {G}iuseppe and {S}ylvestre, {I}sabelle and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, {PVS}2 and {PVS}3. {F}or both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone {H}70-144 and 53% for clone {CP}68-1026, compared with droplet-vitrification in which recovery was 33-37% for clone {H}70-144 and 20-27% for clone {CP}68-1026. {O}ptimal conditions included preculture of encapsulated shoot apices for 2411 in liquid medium with 0.75 {M} sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. {W}ith both protocols employed, regrowth of cryopreserved samples, as followed by visual observation, was always rapid and direct.},
  keywords = {{S}ugarcane ; {C}ryopreservation ; {E}ncapsulation-dehydration ; {D}roplet-vitrification},
  booktitle = {},
  journal = {{S}cientia {H}orticulturae},
  volume = {130},
  numero = {1},
  pages = {320--324},
  ISSN = {0304-4238},
  year = {2011},
  DOI = {10.1016/j.scienta.2011.07.003},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053842},
}