Barraco Giuseppe auteur aut IRD Sylvestre Isabelle auteur aut IRD Iapichino G. auteur aut IRD Engelmann Florent auteur aut IRD text journalArticle eng text/pdf born digital access In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation. specialized Cryopreservation Droplet-vitrification Limonium serotinum Statice Sucrose pretreatment Vitrification solution 076 084 130 1 309-313 2011 0304-4238 https://www.documentation.ird.fr/hor/fdi:010053841 10.1016/j.scienta.2011.07.001 0304-4238 [F B010053841] https://www.documentation.ird.fr/hor/fdi:010053841 https://www.documentation.ird.fr/intranet/publi/2011/10/010053841.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-11-07 2024-04-29 fdi:010053841 fre