@article{fdi:010053839,
  title = {{D}roplet-vitrification of apical shoot tips of {R}ubus fruticosus {L}. and {P}runus cerasifera {E}hrh},
  author = {{V}ujovic, {T}. and {S}ylvestre, {I}sabelle and {R}uzic, {D}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A}pical shoot tips excised from in vitro plantlets of blackberry ({R}ubus fruticosus {L}. '{C}acanska {B}estrna') and cherry plum ({P}runus cerasifera {E}hrh.) were tested for recovery after cryopreservation using the droplet-vitrification technique. {F}ollowing treatment for 30 min with a loading solution comprising 1.9 {M} glycerol and 0.5 {M} sucrose, explants were dehydrated with a highly concentrated cryoprotectant solution, so called vitrification solution. {S}hoot tips were dehydrated for 10,20 and 30 min at room temperature with a solution derived from the original {PVS}2 solution (containing 37.5% (w/v) glycerol, 15% (w/v) dimethylsulfoxide, 15% (w/v) ethylene glycol and 22.5% (w/v) sucrose) and for 60,90 and 120 min using the {PVS}3 solution (containing 50% (w/v) glycerol and 50% (w/v) sucrose). {E}xplants were cooled by direct immersion in {LN} in 10 mu l droplets of vitrification solution placed on aluminium foil strips. {R}ewarming was done by direct plunging of foil strips in a preheated (37 degrees {C}) unloading solution (0.8 {M} sucrose) for 30 s, after which an equal volume of unloading solution (at room temperature) was added for further incubation for 30 min. {A}s for regrowth of blackberry, {PVS}3 proved more effective than the modified {PVS}2, but the difference was significant ({P} < 0.05) only for the shortest treatment duration. {T}he duration of {PVS}3 treatment had no significant effect on regrowth of cryopreserved shoot tips (45.8-70%). {B}y contrast, a 30-min treatment with modified {PVS}2 solution resulted in a significant increase in regeneration percentage (30%), as compared with a 10-min treatment with the same solution (5%). {C}herry plum shoot tips were very sensitive to both vitrification solutions and growth recovery of cryopreserved samples was generally lower (5-20%) than that of blackberry explants. {N}o significant influence of {PVS} treatment (both type of solution and treatment duration) on regrowth of cryopreserved shoot tips was observed with cherry plum shoot tips. {E}xperiments performed in {F}rance and in {S}erbia produced similar results, thereby showing the robustness and reproducibility of the protocols developed.},
  keywords = {{B}lackberry ; {C}herry plum ; {I}n vitro shoot tips ; {D}ehydration ; {V}itrification solution ; {C}ryopreservation},
  booktitle = {},
  journal = {{S}cientia {H}orticulturae},
  volume = {130},
  numero = {1},
  pages = {222--228},
  ISSN = {0304-4238},
  year = {2011},
  DOI = {10.1016/j.scienta.2011.06.049},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053839},
}