Gales G. auteur aut IRD Chehider N. auteur aut IRD Joulian C. auteur aut IRD Battaglia-Brunet F. auteur aut IRD Cayol Jean-Luc auteur aut IRD Postec A. auteur aut IRD Borgomano J. auteur aut IRD Neria-Gonzalez I. auteur aut IRD Lomans B. P. auteur aut IRD Ollivier Bernard auteur aut IRD Alazard Didier auteur aut IRD text journalArticle eng text/pdf born digital access An anaerobic, halophilic, and fermentative bacterium, strain S200(T), was isolated from a core sample of a deep hypersaline oil reservoir. Cells were rod-shaped, non-motile, and stained Gram-positive. It grew at NaCl concentrations ranging from 6 to 26% (w/v), with optimal growth at 15% (w/v) NaCl, and at temperatures between 25 and 47A degrees C with an optimum at 40-45A degrees C. The optimum pH was 7.3 (range 6.2-8.8; no growth at pH 5.8 and pH 9). The doubling time in optimized growth conditions was 3.5 h. Strain S200(T) used exclusively carbohydrates as carbon and energy sources. The end products of glucose degradation were lactate, formate, ethanol, acetate, H-2, and CO2. The predominant cellular fatty acids were non-branched fatty acids C-16:1, C-16:0, and C-14:0. The G + C mole% of the DNA was 32.7%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain S200(T) formed a distinct lineage within the family Halobacteroidaceae, order Halanaerobiales, and was most closely related to Halanaerobaculum tunisiense DSM 19997(T) and Halobacteroides halobius DSM 5150(T), with sequence similarity of 92.3 and 91.9%, respectively. On the basis of its physiological and genotypic properties, strain S200(T) is proposed to be assigned to a novel species of a novel genus, for which the name Halanaerocella petrolearia is proposed. The type strain of Halanaerocella petrolearia is strain S200(T) (=DSM 22693(T) = JCM 16358(T)). specialized Anaerobic Halophilic Taxonomy Halanaerocella petrolearia Oil reservoir 084 15 5 565-571 2011 1431-0651 https://www.documentation.ird.fr/hor/fdi:010053796 10.1007/s00792-011-0387-y 1431-0651 [F B010053796] https://www.documentation.ird.fr/hor/fdi:010053796 https://www.documentation.ird.fr/intranet/publi/2011/09/010053796.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-10-06 2024-04-29 fdi:010053796 fre