@article{fdi:010053785,
  title = {{T}ranscript profiling of crown rootless1 mutant stem base reveals new elements associated with crown root development in rice},
  author = {{C}oudert, {Y}. and {B}{\`e}s, {M}. and {T}hi {V}an {A}nh {L}e, and {P}r{\'e}, {M}. and {G}uiderdoni, {E}. and {G}antet, {P}ascal},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {I}n rice, the major part of the post-embryonic root system is made of stem-derived roots named crown roots ({CR}). {A}mong the few characterized rice mutants affected in root development, crown rootless1 mutant is unable to initiate crown root primordia. {CROWN} {ROOTLESS}1 ({CRL}1) is induced by auxin and encodes an {AS}2/{LOB}-domain transcription factor that acts upstream of the gene regulatory network controlling {CR} development. {R}esults: {T}o identify genes involved in {CR} development, we compared global gene expression profile in stem bases of crl1 mutant and wild-type ({WT}) plants. {O}ur analysis revealed that 250 and 236 genes are down- and up-regulated respectively in the crl1 mutant. {A}uxin induces {CRL}1 expression and consequently it is expected that auxin also alters the expression of genes that are early regulated by {CRL}1. {T}o identify genes under the early control of {CRL}1, we monitored the expression kinetics of a selected subset of genes, mainly chosen among those exhibiting differential expression, in crl1 and {WT} following exogenous auxin treatment. {T}his analysis revealed that most of these genes, mainly related to hormone, water and nutrient, development and homeostasis, were likely not regulated directly by {CRL}1. {W}e hypothesized that the differential expression for these genes observed in the crl1 mutant is likely a consequence of the absence of {CR} formation. {O}therwise, three {CRL}1-dependent auxin-responsive genes: {FSM} ({FLATENNED} {SHOOT} {MERISTEM})/{FAS}1 ({FASCIATA}1), {GTE}4 ({GENERAL} {TRANSCRIPTION} {FACTOR} {GROUP} {E}4) and {MAP} ({MICROTUBULE}-{ASSOCIATED} {PROTEIN}) were identified. {FSM}/{FAS}1 and {GTE}4 are known in rice and {A}rabidopsis to be involved in the maintenance of root meristem through chromatin remodelling and cell cycle regulation respectively. {C}onclusion: {O}ur data showed that the differential regulation of most genes in crl1 versus {WT} may be an indirect consequence of {CRL}1 inactivation resulting from the absence of {CR} in the crl1 mutant. {N}evertheless some genes, {FAS}1/{FSM}, {GTE}4 and {MAP}, require {CRL}1 to be induced by auxin suggesting that they are likely directly regulated by {CRL}1. {T}hese genes have a function related to polarized cell growth, cell cycle regulation or chromatin remodelling. {T}his suggests that these genes are controlled by {CRL}1 and involved in {CR} initiation in rice.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {G}enomics},
  volume = {12},
  numero = {},
  pages = {387},
  ISSN = {1471-2164},
  year = {2011},
  DOI = {10.1186/1471-2164-12-387},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053785},
}