Auguy Florence auteur aut IRD Abdel-Lateif K. auteur aut IRD Doumas Patrick auteur aut IRD Badin P. auteur aut IRD Guerin V. auteur aut IRD Bogusz Didier auteur aut IRD Hocher Valérie auteur aut IRD text journalArticle eng text/pdf born digital access We investigated the involvement of flavonoids in the actinorhizal nodulation process resulting from the interaction between the tropical tree Casuarina glauca Sieb. ex Spreng. and the actinomycete Frankia. Eight C. glauca genes involved in flavonoid biosynthesis: chalcone synthase (CHS), chalcone isomerase (CHI), isoflavone reductase (IFR), flavonoid-3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonoid 3',5' hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR) and flavonol synthase (FLS), were identified from a unigene database and gene expression patterns were monitored by quantitative real-time PCR (qRT-PCR) during the nodulation time course. Results showed that FLS and F3'5'H transcripts accumulated in mature nodules whereas CHI and IFR transcripts accumulated preferentially early after inoculation with Frankia. Comparison of IFR and CHI expression in inoculated plants and in control plants cultivated with or without nitrogen confirmed that early expression of IFR is specifically linked to symbiosis. Taken together, these data suggest for the first time that isoflavonoids are implicated in actinorhizal nodulation. specialized actinorhiza flavonoid gene expression isoflavonoid nitrogen-fixing symbiosis 084 076 38 8-9 690-696 2011 1445-4408 https://www.documentation.ird.fr/hor/fdi:010053775 10.1071/fp11014 1445-4408 [F B010053775] https://www.documentation.ird.fr/hor/fdi:010053775 https://www.documentation.ird.fr/intranet/publi/2011/09/010053775.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-10-06 2017-08-23 fdi:010053775 fre