%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gomez-Bonilla, Y.
%A Lopez-Ferber, M.
%A Caballero, P.
%A Léry, Xavier
%A Munoz, D.
%T Characterization of a Costa Rican granulovirus strain highly pathogenic against its indigenous hosts, Phthorimaea operculella and Tecia solanivora
%D 2011
%L fdi:010053740
%G ENG
%J Entomologia Experimentalis et Applicata
%@ 0013-8703
%K microbial control ; adaptation to host ; PhopGV ; Baculoviridae ; Lepidoptera ; Gelechiidae ; tuberworm ; integrated pest management ; Betabaculovirus
%M ISI:000293525000007
%N 3
%P 238-246
%R 10.1111/j.1570-7458.2011.01156.x
%U https://www.documentation.ird.fr/hor/fdi:010053740
%> https://www.documentation.ird.fr/intranet/publi/2011/08/010053740.pdf
%V 140
%W Horizon (IRD)
%X A granulovirus isolate collected from diseased Phthorimaea operculella (Zeller) (Lepidoptera: Gele-chiidae) larvae in Costa Rican potato [Solanum tuberosum L. (Solanaceae)] crops was characterized at the molecular and biological level. Restriction endonuclease analysis identified this isolate as a novel P. operculella granulovirus (PhopGV) (Baculoviridae: Betabaculovirus) strain and was designated as PhopGV-CR1. In addition, PCR amplification of four specific variable genomic regions yielded multiple amplicons for two open reading frames, revealing the presence of different genotypic variants within the virus population. Biologically, PhopGV-CR1 was highly pathogenic for its two indigenous hosts, although significant differences of up to four-fold were detected against P. operculella [LD50 = 17.9 occlusion bodies (OBs) mm)(-2)] and Tecia solanivora (Povolny) (Lepidoptera: Gele-chiidae) (LD50 = 69.1 OBs mm)(-2)). The two P. operculella colonies, from Costa Rica and France, were equally susceptible to PhopGV-CR1. Serial passage of PhopGV-CR1 over four generations in T. solanivora increased its pathogenicity by five-fold in three generations, suggesting an ongoing adaptation to its alternate host.
%$ 076