@article{fdi:010053711,
  title = {{G}enetic diversity of symbiotic {B}radyrhizobium elkanii populations recovered from inoculated and non-inoculated {A}cacia mangium field trials in {B}razil},
  author = {{P}errineau, {M}. {M}. and {L}e {R}oux, {C}. and de {F}aria, {S}. {M}. and de {C}arvalho {B}alieiro, {F}. and {G}aliana, {A}. and {P}rin, {Y}. and {B}{\'e}na, {G}illes},
  editor = {},
  language = {{ENG}},
  abstract = {{A}cacia mangium is a legume tree native to {A}ustralasia. {S}ince the eighties, it has been introduced into many tropical countries, especially in a context of industrial plantations. {M}any field trials have been set up to test the effects of controlled inoculation with selected symbiotic bacteria versus natural colonization with indigenous strains. {I}n the introduction areas. {A}. mangium trees spontaneously nodulate with local and often ineffective bacteria. {W}hen inoculated, the persistence of inoculants and possible genetic recombination with local strains remain to be explored. {T}he aim of this study was to describe the genetic diversity of bacteria spontaneously nodulating {A}. mangium in {B}razil and to evaluate the persistence of selected strains used as inoculants. {T}hree different sites, several hundred kilometers apart, were studied, with inoculated and non-inoculated plots in two of them. {S}eventy-nine strains were isolated from nodules and sequenced on three housekeeping genes (gln{II}, dna{K} and rec{A}) and one symbiotic gene (nod{A}). {A}ll but one of the strains belonged to the {B}radyrhizobium elkanii species. {A} single case of housekeeping gene transfer was detected among the 79 strains, suggesting an extremely low rate of recombination within {B}. elkanii, whereas the nodulation gene nod{A} was found to be frequently transferred. {T}he fate of the inoculant strains varied depending on the site, with a complete disappearance in one case, and persistence in another. {W}e compared our results with the sister species {B}radyrhizobium japonicum, both in terms of population genetics and inoculant strain destiny.},
  keywords = {{N}itrogen fixation ; {C}ontrolled inoculation ; {M}olecular systematics ; {R}ecombination ; {A}cacia mangium ; {B}radyrhizobium ; {B}razil},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {34},
  numero = {5},
  pages = {376--384},
  ISSN = {0723-2020},
  year = {2011},
  DOI = {10.1016/j.syapm.2011.03.003},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053711},
}