@article{fdi:010053690,
  title = {{B}acterial {R}u{B}is{CO} is required for efficient {B}radyrhizobium/{A}eschynomene symbiosis},
  author = {{G}ourion, {B}. and {D}elmotte, {N}. and {B}onaldi, {K}. and {N}ouwen, {N}ico and {V}orholt, {J}. {A}. and {G}iraud, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{R}hizobia and legume plants establish symbiotic associations resulting in the formation of organs specialized in nitrogen fixation. {I}n such organs, termed nodules, bacteria differentiate into bacteroids which convert atmospheric nitrogen and supply the plant with organic nitrogen. {A}s a counterpart, bacteroids receive carbon substrates from the plant. {T}his rather simple model of metabolite exchange underlies symbiosis but does not describe the complexity of bacteroids' central metabolism. {A} previous study using the tropical symbiotic model {A}eschynomene indica/photosynthetic {B}radyrhizobium sp. {ORS}278 suggested a role of the bacterial {C}alvin cycle during the symbiotic process. {H}erein we investigated the role of two {R}u{B}is{CO} gene clusters of {B}radyrhizobium sp. {ORS}278 during symbiosis. {U}sing gene reporter fusion strains, we showed that cbb{L}1 but not the paralogous cbb{L}2 is expressed during symbiosis. {C}ongruently, {C}bb{L}1 was detected in bacteroids by proteome analysis. {T}he importance of {C}bb{L}1 for symbiotic nitrogen fixation was proven by a reverse genetic approach. {I}nterestingly, despite its symbiotic nitrogen fixation defect, the cbb{L}1 mutant was not affected in nitrogen fixation activity under free living state. {T}his study demonstrates a critical role for bacterial {R}u{B}is{CO} during a rhizobia/legume symbiotic interaction.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {6},
  numero = {7},
  pages = {e21900},
  ISSN = {1932-6203},
  year = {2011},
  DOI = {10.1371/journal.pone.0021900},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053690},
}