@article{fdi:010053687,
  title = {{M}olecular epidemiology of malaria in {C}ameroon. {XXX}. {S}equence analysis of {P}lasmodium falciparum {ATP}ase 6, dihydrofolate reductase, and dihydropteroate synthase resistance markers in clinical isolates from children treated with an artesunate-sulfadoxine-pyrimethamine combination},
  author = {{M}enemedengue, {V}. and {S}ahnouni, {K}. and {B}asco, {L}eonardo and {T}ahar, {R}achida},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lasmodium falciparum dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) genes are reliable molecular markers for antifolate resistance. {T}he {P}. falciparum {ATP}ase 6 (pfatp6) gene has been proposed to be a potential marker for artemisinin resistance. {I}n our previous clinical study, we showed that artesunate-sulfadoxine-pyrimethamine is highly effective against uncomplicated malaria in {Y}aounde, {C}ameroon. {I}n the present study, dhfr, dhps, and pfatp6 mutations in {P} falciparum isolates obtained from children treated with artesunate-sulfadoxine-pyrimethamine were determined. {A}ll 61 isolates had wild-type {P}fatp6 263, 623, and 769 alleles, and 11(18%) had a single {E}431{K} substitution. {T}hree additional mutations, {E}643{Q}, {E}432{K}, and {E}641{Q}, were detected. {T}he results did not indicate any warning signal of serious concern (i.e., no parasites were seen with quintuple dhfr-dhps, {DHFR} {I}le164{L}eu, or pfatp6 mutations), as confirmed by the high clinical efficacy of artesunate-sulfadoxine-pyrimethamine. {F}urther studies are required to identify a molecular marker that reliably predicts artemisinin resistance.},
  keywords = {},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {85},
  numero = {1},
  pages = {22--25},
  ISSN = {0002-9637},
  year = {2011},
  DOI = {10.4269/ajtmh.2011.10-0523},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053687},
}