@article{fdi:010053673,
  title = {{C}ytogenetic map for {A}nopheles nili : application for population genetics and comparative physical mapping},
  author = {{S}harakhova, {M}. {V}. and {A}ntonio-{N}kondjio, {C}. and {X}ia, {A}. and {N}do, {C}. and {A}wono-{A}mbene, {P}. and {S}imard, {F}r{\'e}d{\'e}ric and {S}harakhov, {I}. {V}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}nopheles nili is one of the major malaria vectors in {A}frica with a wide geographic distribution. {H}owever, the taxonomic and population genetic studies on this species are scarce. {N}ew research tools are urgently needed to genetically characterize this important malaria vector. {I}n this study, a high-resolution cytogenetic map was developed for {A}n. nili polytene chromosomes. {C}hromosomes were straightened and subdivided into 46 numbered divisions according to the banding pattern. {P}opulation analysis of {A}n. nili females collected in {B}urkina {F}aso revealed the presence of two highly polymorphic inversions on the 2{R} chromosomal arm. {A} statistically significant departure from {H}ardy-{W}einberg equilibrium due to a deficit in heterozygotes was detected for inversion 2{R}b. {T}o determine chromosome homologies and gene order conservation between {A}n. nili and other major malaria vectors, {PCR} probes based on the {A}n. gambiae coding sequences were mapped to {A}n. nili chromosomes. {C}omparative mapping demonstrated that {A}n. nili chromosomes have an {A}n. stephensi-like arm association and that whole-arm translocations and paracentric inversions were the major types of rearrangement in evolution of these mosquitoes. {T}he minimum number of fixed inversions among {A}n. nili, {A}n. gambiae, and {A}n. stephensi was calculated using the {M}ultiple {G}enome {R}earrangements ({MGR}), {G}enome {R}earrangements {I}n {M}an and {M}ouse ({GRIMM}), and {S}orting {P}ermutation by {R}eversals and block-{IN}terchan{G}es ({SPRING}) programs. {T}he data suggest that the {A}n. nili is, at least, as diverged from {A}n. gambiae as {A}n. stephensi. {W}e provide evidence that 2{L}a/a arrangement of {A}n. gambiae is present in outgroup species {A}n. nili and {A}n. stephensi confirming the ancestral status of the 2{L}a inversion in the {A}n. gambiae complex. {A}vailability of the new polytene chromosome map, polymorphic inversions, and physically mapped {DNA} markers for {A}n. nili will further stimulate population genetic, taxonomic, and genomic studies of this neglected malaria vector.},
  keywords = {{M}alaria mosquito ; {A}nopheles nili ; {A}nopheles gambiae ; {A}nopheles ; stephensi ; {P}hysical mapping ; {P}olytene chromosomes ; {I}nversion ; {F}luorescent in situ hybridization},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {11},
  numero = {4},
  pages = {746--754},
  ISSN = {1567-1348},
  year = {2011},
  DOI = {10.1016/j.meegid.2010.06.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053673},
}