@article{fdi:010053663,
  title = {{H}igh-throughput microsatellite isolation through 454 {GS}-{FLX} titanium pyrosequencing of enriched {DNA} libraries},
  author = {{M}alausa, {T}. and {G}illes, {A}. and {M}eglecz, {E}. and {B}lanquart, {H}. and {D}uthoy, {S}. and {C}ostedoat, {C}. and {D}ubut, {V}. and {P}ech, {N}. and {C}astagnone-{S}ereno, {P}. and {D}{\'e}lye, {C}. and {F}eau, {N}. and {F}rey, {P}. and {G}authier, {P}hilippe and {G}uillemaud, {T}. and {H}azard, {L}. and {L}e {C}orre, {V}. and {L}ung-{E}scarmant, {B}. and {M}al{\'e}, {P}. {J}. {G}. and {F}erreira, {S}. and {M}artin, {J}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}icrosatellites (or {SSR}s: simple sequence repeats) are among the most frequently used {DNA} markers in many areas of research. {T}he use of microsatellite markers is limited by the difficulties involved in their de novo isolation from species for which no genomic resources are available. {W}e describe here a high-throughput method for isolating microsatellite markers based on coupling multiplex microsatellite enrichment and next-generation sequencing on 454 {GS}-{FLX} {T}itanium platforms. {T}he procedure was calibrated on a model species ({A}pis mellifera) and validated on 13 other species from various taxonomic groups (animals, plants and fungi), including taxa for which severe difficulties were previously encountered using traditional methods. {W}e obtained from 11 497 to 34 483 sequences depending on the species and the number of detected microsatellite loci ranged from 199 to 5791. {W}e thus demonstrated that this procedure can be readily and successfully applied to a large variety of taxonomic groups, at much lower cost than would have been possible with traditional protocols. {T}his method is expected to speed up the acquisition of high-quality genetic markers for nonmodel organisms.},
  keywords = {enriched library ; genetic marker ; genotyping ; microsatellite isolation ; next-generation sequencing ; primer design ; pyrosequencing},
  booktitle = {},
  journal = {{M}olecular {E}cology {R}esources},
  volume = {11},
  numero = {4},
  pages = {638--644},
  ISSN = {1755-098{X}},
  year = {2011},
  DOI = {10.1111/j.1755-0998.2011.02992.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053663},
}