@article{fdi:010053636,
  title = {{E}xpression and trans-specific polymorphism of self-incompatibility {RN}ases in {C}offea ({R}ubiaceae)},
  author = {{N}owak, {M}. {D}. and {D}avis, {A}. {P}. and {A}nthony, {F}ran{\c{c}}ois and {Y}oder, {A}. {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}elf-incompatibility ({SI}) is widespread in the angiosperms, but identifying the biochemical components of {SI} mechanisms has proven to be difficult in most lineages. {C}offea (coffee; {R}ubiaceae) is a genus of old-world tropical understory trees in which the vast majority of diploid species utilize a mechanism of gametophytic self-incompatibility ({GSI}). {T}he {S}-{RN}ase {GSI} system was one of the first {SI} mechanisms to be biochemically characterized, and likely represents the ancestral {E}udicot condition as evidenced by its functional characterization in both asterid ({S}olanaceae, {P}lantaginaceae) and rosid ({R}osaceae) lineages. {T}he {S}-{RN}ase {GSI} mechanism employs the activity of class {III} {RN}ase {T}2 proteins to terminate the growth of "self" pollen tubes. {H}ere, we investigate the mechanism of {C}offea {GSI} and specifically examine the potential for homology to {S}-{RN}ase {GSI} by sequencing class {III} {RN}ase {T}2 genes in populations of 14 {A}frican and {M}adagascan {C}offea species and the closely related self-compatible species {P}silanthus ebracteolatus. {P}hylogenetic analyses of these sequences aligned to a diverse sample of plant {RN}ase {T}2 genes show that the {C}offea genome contains at least three class {III} {RN}ase {T}2 genes. {P}atterns of tissue-specific gene expression identify one of these {RN}ase {T}2 genes as the putative {C}offea {S}-{RN}ase gene. {W}e show that populations of {SI} {C}offea are remarkably polymorphic for putative {S}-{RN}ase alleles, and exhibit a persistent pattern of trans-specific polymorphism characteristic of all {S}-{RN}ase genes previously isolated from {GSI} {E}udicot lineages. {W}e thus conclude that {C}offea {GSI} is most likely homologous to the classic {E}udicot {S}-{RN}ase system, which was retained since the divergence of the {R}ubiaceae lineage from an ancient {SI} {E}udicot ancestor, nearly 90 million years ago.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {6},
  numero = {6},
  pages = {e21019},
  ISSN = {1932-6203},
  year = {2011},
  DOI = {10.1371/journal.pone.0021019},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053636},
}