%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Garrigos, Y. E.
%A Lambourdière, J.
%A Ibanez Luna, C.
%A Gaubert, Philippe
%T Characterization of ten polymorphic microsatellite loci in the Andean pupfish Orestias agassizii, with cross-amplification in the sympatric O. luteus
%D 2011
%L fdi:010053558
%G ENG
%J Conservation Genetics Resources
%@ 1877-7252
%K Cyprinodontidae ; Orestias agassizii ; Andean altiplano ; Microsatellites ; Heterozygosity ; Cross-amplification ; Orestias luteus
%M ISI:000290438500005
%N 1
%P 17-19
%R 10.1007/s12686-010-9269-3
%U https://www.documentation.ird.fr/hor/fdi:010053558
%> https://www.documentation.ird.fr/intranet/publi/2011/06/010053558.pdf
%V 3
%W Horizon (IRD)
%X We describe the isolation and characterization of ten polymorphic microsatellite loci in the Andean pupfish Orestias agassizii. The number of alleles per locus in 25 individuals was high, varying from 5 to 25. Observed and expected mean heterozygosities were 0.750 and 0.767, respectively. The ten loci conformed to the Hardy-Weinberg equilibrium, and we did not detect linkage disequilibrium between loci. Cross-species amplification in O. luteus was entirely successful, and polymorphism was detected in all loci. One locus (A116) showed significant departure from Hardy-Weinberg equilibrium due to homozygote excess. These microsatellites will be useful for assessing the genetic structure and historical demography of Andean pupfishes, which are subject to a global collapse of their stocks.
%$ 034