Bruneel Odile auteur aut IRD Volant A. auteur aut IRD Gallien S. auteur aut IRD Chaumande B. auteur aut IRD Casiot C. auteur aut IRD Carapito C. auteur aut IRD Bardil A. auteur aut IRD Morin G. auteur aut IRD Brown G. E. auteur aut IRD Personne C. J. auteur aut IRD Le Paslier D. auteur aut IRD Schaeffer C. auteur aut IRD Van Dorsselaer A. auteur aut IRD Bertin P. N. auteur aut IRD Elbaz Poulichet F. auteur aut IRD Arsene Ploetze F. auteur aut IRD text journalArticle eng text/pdf born digital access Acid mine drainage of the CarnoulSs mine (France) is characterized by acid waters containing high concentrations of arsenic and iron. In the first 30 m along the Reigous, a small creek draining the site, more than 38% of the dissolved arsenic was removed by co-precipitation with Fe(III), in agreement with previous studies, which suggest a role of microbial activities in the co-precipitation of As(III) and As(V) with Fe(III) and sulfate. To investigate how this particular ecosystem functions, the bacterial community was characterized in water and sediments by 16S rRNA encoding gene library analysis. Based on the results obtained using a metaproteomic approach on sediments combined with high-sensitivity HPLC-chip spectrometry, several GroEL orthologs expressed by the community were characterized, and the active members of the prokaryotic community inhabiting the creek sediments were identified. Many of these bacteria are beta-proteobacteria such as Gallionella and Thiomonas, but gamma-proteobacteria such as Acidithiobacillus ferrooxidans and alpha-proteobacteria such as Acidiphilium, Actinobacteria, and Firmicutes were also detected. specialized 036 61 4 793-810 2011 0095-3628 https://www.documentation.ird.fr/hor/fdi:010053555 10.1007/s00248-011-9808-9 0095-3628 [F B010053555] https://www.documentation.ird.fr/hor/fdi:010053555 https://www.documentation.ird.fr/intranet/publi/2011/06/010053555.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-07-01 2022-02-22 fdi:010053555 fre