@article{fdi:010053528,
  title = {{P}henol biodegradation by the thermoacidophilic archaeon {S}ulfolobus solfataricus 98/2 in a fed-batch bioreactor},
  author = {{C}hristen, {P}ierre and {D}avidson, {S}ylvain and {C}ombet-{B}lanc, {Y}annick and {A}uria, {R}ichard},
  editor = {},
  language = {{ENG}},
  abstract = {{T}oxic at low concentrations, phenol is one of the most common organic pollutants in air and water. {I}n this work, phenol biodegradation was studied in extreme conditions (80{A} degrees {C}, p{H} = 3.2) in a 2.7 l bioreactor with the thermoacidophilic archaeon {S}ulfolobus solfataricus 98/2. {T}he strain was first acclimatized to phenol on a mixture of glucose (2000 mg l(-1)) and phenol (94 mg l(-1)) at a constant dissolved oxygen concentration of 1.5 mg l(-1). {A}fter a short lag-phase, only glucose was consumed. {P}henol degradation then began while glucose was still present in the reactor. {W}hen glucose was exhausted, phenol was used for respiration and then for biomass build-up. {A}fter several batch runs (phenol < 365 mg l(-1)), specific growth rate (mu({X})) was 0.034 +/- {A} 0.001 h(-1), specific phenol degradation rate (q({P})) was 57.5 +/- {A} 2 mg g(-1) h(-1), biomass yield ({Y}-{X}/{P}) was 52.2 +/- {A} 1.1 g mol(-1), and oxygen yield factor ({Y}-x/o2) was 9.2 +/- {A} 0.2 g mol(-1). {A} carbon recovery close to 100% suggested that phenol was exclusively transformed into biomass (35%) and {CO}2 (65%). {M}olar phenol oxidation constant ({Y}-o2/{P}) was calculated from stoichiometry of phenol oxidation and introducing experimental biomass and {CO}2 conversion yields on phenol, leading to values varying between 4.78 and 5.22 mol mol(-1). {R}espiratory quotient was about 0.84 mol mol(-1), very close to theoretical value (0.87 mol mol(-1)). {C}arbon dioxide production, oxygen demand and redox potential, monitored on-line, were good indicators of growth, substrate consumption and exhaustion, and can therefore be usefully employed for industrial phenol bioremediation in extreme environments.},
  keywords = {{S}ulfolobus solfataricus ; {P}henol ; {K}inetics ; {B}iodegradation ; {Y}ields},
  booktitle = {},
  journal = {{B}iodegradation},
  volume = {22},
  numero = {3},
  pages = {475--484},
  ISSN = {0923-9820},
  year = {2011},
  DOI = {10.1007/s10532-010-9420-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053528},
}