Sabot François auteur aut IRD Picault N. auteur aut IRD El-Baidouri M. auteur aut IRD Llauro C. auteur aut IRD Chaparro C. auteur aut IRD Piegu B. auteur aut IRD Roulin A. auteur aut IRD Guiderdoni E. auteur aut IRD Delabastide M. auteur aut IRD McCombie R. auteur aut IRD Panaud O. auteur aut IRD text journalArticle eng text/pdf born digital access Transposable elements (TEs) are mobile entities that densely populate most eukaryotic genomes and contribute to both their structural and functional dynamics. However, most TE-related sequences in both plant and animal genomes correspond to inactive, degenerated elements, due to the combined effect of silencing pathways and elimination through deletions. One of the major difficulties in fully characterizing the molecular basis of genetic diversity of a given species lies in establishing its genome-wide transpositional activity. Here, we provide an extensive survey of the transpositional landscape of a plant genome using a deep sequencing strategy. This was achieved through paired-end mapping of a fourfold coverage of the genome of rice mutant line derived from an in vitro callus culture using Illumina technology. Our study shows that at least 13 TE families are active in this genotype, causing 34 new insertions. This next-generation sequencing-based strategy provides new opportunities to quantify the impact of TEs on the genome dynamics of the species. specialized rice transposable elements next-generation sequencing genomics mutant line paired-end mapping 076 66 2 241-246 2011 0960-7412 https://www.documentation.ird.fr/hor/fdi:010053480 10.1111/j.1365-313X.2011.04492.x 0960-7412 [F B010053480] https://www.documentation.ird.fr/hor/fdi:010053480 https://www.documentation.ird.fr/intranet/publi/2011/05/010053480.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-05-31 2017-08-23 fdi:010053480 fre