@article{fdi:010053480, title = {{T}ranspositional landscape of the rice genome revealed by paired-end mapping of high-throughput re-sequencing data}, author = {{S}abot, {F}ran{\c{c}}ois and {P}icault, {N}. and {E}l-{B}aidouri, {M}. and {L}lauro, {C}. and {C}haparro, {C}. and {P}iegu, {B}. and {R}oulin, {A}. and {G}uiderdoni, {E}. and {D}elabastide, {M}. and {M}c{C}ombie, {R}. and {P}anaud, {O}.}, editor = {}, language = {{ENG}}, abstract = {{T}ransposable elements ({TE}s) are mobile entities that densely populate most eukaryotic genomes and contribute to both their structural and functional dynamics. {H}owever, most {TE}-related sequences in both plant and animal genomes correspond to inactive, degenerated elements, due to the combined effect of silencing pathways and elimination through deletions. {O}ne of the major difficulties in fully characterizing the molecular basis of genetic diversity of a given species lies in establishing its genome-wide transpositional activity. {H}ere, we provide an extensive survey of the transpositional landscape of a plant genome using a deep sequencing strategy. {T}his was achieved through paired-end mapping of a fourfold coverage of the genome of rice mutant line derived from an in vitro callus culture using {I}llumina technology. {O}ur study shows that at least 13 {TE} families are active in this genotype, causing 34 new insertions. {T}his next-generation sequencing-based strategy provides new opportunities to quantify the impact of {TE}s on the genome dynamics of the species.}, keywords = {rice ; transposable elements ; next-generation sequencing ; genomics ; mutant line ; paired-end mapping}, booktitle = {}, journal = {{P}lant {J}ournal}, volume = {66}, numero = {2}, pages = {241--246}, ISSN = {0960-7412}, year = {2011}, DOI = {10.1111/j.1365-313{X}.2011.04492.x}, URL = {https://www.documentation.ird.fr/hor/fdi:010053480}, }