@article{fdi:010053458,
  title = {{C}omparative genomics reveals diversity among xanthomonads infecting tomato and pepper},
  author = {{P}otnis, {N}. and {K}rasileva, {K}. and {C}how, {V}. and {A}lmeida, {N}. {F}. and {P}atil, {P}. {B}. and {R}yan, {R}. {P}. and {S}harlach, {M}. and {B}ehlau, {F}. and {D}ow, {J}. {M}. and {M}omol, {M}. {T}. and {W}hite, {F}. {F}. and {P}reston, {J}. {F}. and {V}inatzer, {B}. {A}. and {K}oebnik, {R}alf and {S}etubal, {J}. {C}. and {N}orman, {D}. {J}. and {S}taskawicz, {B}. {J}. and {J}ones, {J}. {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {B}acterial spot of tomato and pepper is caused by four {X}anthomonas species and is a major plant disease in warm humid climates. {T}he four species are distinct from each other based on physiological and molecular characteristics. {T}he genome sequence of strain 85-10, a member of one of the species, {X}anthomonas euvesicatoria ({X}cv) has been previously reported. {T}o determine the relationship of the four species at the genome level and to investigate the molecular basis of their virulence and differing host ranges, draft genomic sequences of members of the other three species were determined and compared to strain 85-10. {R}esults: {W}e sequenced the genomes of {X}. vesicatoria ({X}v) strain 1111 ({ATCC} 35937), {X}. perforans ({X}p) strain 91-118 and {X}. gardneri ({X}g) strain 101 ({ATCC} 19865). {T}he genomes were compared with each other and with the previously sequenced {X}cv strain 85-10. {I}n addition, the molecular features were predicted that may be required for pathogenicity including the type {III} secretion apparatus, type {III} effectors, other secretion systems, quorum sensing systems, adhesins, extracellular polysaccharide, and lipopolysaccharide determinants. {S}everal novel type {III} effectors from {X}g strain 101 and {X}v strain 1111 genomes were computationally identified and their translocation was validated using a reporter gene assay. {A} homolog to {A}x21, the elicitor of {XA}21-mediated resistance in rice, and a functional {A}x21 sulfation system were identified in {X}cv. {G}enes encoding proteins with functions mediated by type {II} and type {IV} secretion systems have also been compared, including enzymes involved in cell wall deconstruction, as contributors to pathogenicity. {C}onclusions: {C}omparative genomic analyses revealed considerable diversity among bacterial spot pathogens, providing new insights into differences and similarities that may explain the diverse nature of these strains. {G}enes specific to pepper pathogens, such as the {O}-antigen of the lipopolysaccharide cluster, and genes unique to individual strains, such as novel type {III} effectors and bacteriocin genes, have been identified providing new clues for our understanding of pathogen virulence, aggressiveness, and host preference. {T}hese analyses will aid in efforts towards breeding for broad and durable resistance in economically important tomato and pepper cultivars.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {G}enomics},
  volume = {12},
  numero = {},
  pages = {146},
  ISSN = {1471-2164},
  year = {2011},
  DOI = {10.1186/1471-2164-12-146},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053458},
}